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European Collection of Authenticated Cell Cultures
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Johns Hopkins HealthCare
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BioResource International Inc
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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Inhibitors of Copi and Copii Do Not Block PEX3 -Mediated Peroxisome Synthesis
doi:
Figure Lengend Snippet: PEX3 encodes a peroxisomal protein. A postnuclear supernatant of HepG2 cells was fractionated by Nycodenz gradient centrifugation. Equal proportions of each fraction were assayed for the marker enzymes catalase (a peroxisomal marker), SDH (a mitochondrial marker), and NCR (a microsomal marker). Equal proportions of each fraction were also assayed by immunoblot with antibodies to PEX3. A 42-kD band corresponding to PEX3 was detected exclusively in the peroxisomal fractions.
Article Snippet:
Techniques: Gradient Centrifugation, Marker, Western Blot